| 產(chǎn)品編號(hào) | bs-3060R |
| 英文名稱 | Phospho-CEBP alpha (Ser21) Rabbit pAb |
| 中文名稱 | 磷酸化轉(zhuǎn)錄調(diào)節(jié)因子CEBP α 抗體 |
| 別 名 | Apoptotic cysteine protease; Apoptotic protease Mch 5; C/EBP alpha; CAP4; Caspase 8 precursor; CCAAT Enhancer Binding Protein alpha; CEBP; CEBP A; CEBP alpha; CEBPA; FADD homologous ICE/CED 3 like protease; FADD like ICE; FLICE; ICE like apoptotic proteas |
| 產(chǎn)品類(lèi)型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow) |
| 產(chǎn)品應(yīng)用 | ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human CDBP alpha around the phosphorylation site of Ser21: LQ(p-S)PP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Mutations in this gene are frequently found in acute myeloid leukemia (AML). Function: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Subunit: Interacts with PRDM16 (By similarity). Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X. Interacts with ZNF638; this interaction increases transcriptional activation. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain. SWISS: P49715 Gene ID: 1050 Database links: Entrez Gene: 1050 Human Entrez Gene: 12606 Mouse Omim: 116897 Human SwissProt: P49715 Human SwissProt: P53566 Mouse Unigene: 76171 Human Unigene: 349667 Mouse Unigene: 153603 Rat Unigene: 204833 Rat |
| 產(chǎn)品圖片 |
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-CEBP alpha (Ser21)) polyclonal Antibody, Unconjugated (bs-3060R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-CEBP alpha (Ser21)) polyclonal Antibody, Unconjugated (bs-3060R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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