| 產(chǎn)品編號 | bs-3158R |
| 英文名稱 | Phospho-FRA1 (Ser265) Rabbit pAb |
| 中文名稱 | 磷酸化FRA-1蛋白抗體 |
| 別 名 | FOSL1; Fos related antigen 1; FOS L1; FOS like antigen 1; FOSL 1; FOSL1 protein; FRA 1; Fos-related antigen 1; FOSL1_HUMAN; FRA-1. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human FRA1 around the phosphorylation site of Ser265: LG(p-S)PT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq]. Subunit: Heterodimer. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. Fos subfamily. Contains 1 bZIP (basic-leucine zipper) domain. SWISS: P15407 Gene ID: 8061 Database links: Entrez Gene: 8061 Human Omim: 136515 Human SwissProt: P15407 Human Unigene: 283565 Human FOSL1蛋白對細胞因子、生長因子、感染或致癌刺激等生理或病理信號發(fā)生應(yīng)答,調(diào)節(jié)基因的轉(zhuǎn)錄, 參與細胞的增殖、分化等過程。 FOSL1在腫瘤形成及發(fā)展過程中, 通過促進細胞增殖、抑制分化、促進腫瘤細胞的侵襲和轉(zhuǎn)移等過程發(fā)揮作用。 |
| 產(chǎn)品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- Phospho-FRA1 (Ser265) (bs-3158R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FRA1 (Ser265)) Polyclonal Antibody, Unconjugated (bs-3158R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-FRA1(Ser265) Polyclonal Antibody, Unconjugated(bs-3158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
