| 產(chǎn)品編號 | bs-2352R |
| 英文名稱 | Cytochrome P450 2B6 Rabbit pAb |
| 中文名稱 | 細胞色素P450 2B6抗體 |
| 別 名 | 11B1; CP2B6_HUMAN; CPB 6; CPB6; CYP 2B6; CYP IIB6; CYP2B; CYP2B6; CYP2B7; CYP2B7P; CYPIIB6; Cytochrome P450 2B6; Cytochrome P450 family 2 subfamily B; Cytochrome P450 family 2 subfamily B polypeptide 6; Cytochrome P450 IIB1; Cytochrome P450 phenobarbital |
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Specific References (1) | bs-2352R has been referenced in 1 publications.
[IF=2.8] Man, Shuli, et al. "Combination therapy of cyclophosphamide and Rhizoma Paridis Saponins on anti-hepatocarcinoma mice and effects on cytochrome p450 enzyme expression." Steroids (2013). WB ; Rat.
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| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 信號轉導 細胞凋亡 轉錄調(diào)節(jié)因子 新陳代謝 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cytochrome P450 2B6: 401-491/491 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Tissue Specificity: Expressed in liver, lung and heart right ventricle. Post-translational modifications: Phosphorylation is accompanied by a decrease in enzyme activity (By similarity). Similarity: Belongs to the cytochrome P450 family. SWISS: P20813 Gene ID: 1555 Database links: Entrez Gene: 1555 Human Omim: 123930 Human SwissProt: P20813 Human Unigene: 1360 Human |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome P450 2B6 (bs-2352R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome P450 2B6 (bs-2352R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Cytochrome P450 2B6 antibody (bs-2352R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
