| 產(chǎn)品編號 | bs-4114R |
| 英文名稱 | IKBKE/IKKi/IKKe Rabbit pAb |
| 中文名稱 | 核因子κB抑制蛋白E抗體 |
| 別 名 | IKKi/IKKe; I kappa B kinase epsilon; IkBKE; IKBKE protein; IKK E; IKK i; IKK-i; IKK related kinase epsilon; IKKE; IKK-E; IKKepsilon; IKKI; Inducible I kappa B kinase; inducible IkappaB kinase; Inhibitor of kappa light polypeptide gene enhancer in B cells |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 79 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IKBKE: 501-600/716 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]. Function: Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death. Subunit: May interact with MAVS/IPS1. Interacts with AZI2. Interacts with SIKE1. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between IKBKE and SIKE1. Interacts with TOPORS; induced by DNA damage. Interacts with CYLD. Subcellular Location: Cytoplasm. Nucleus. Nucleus, PML body. Tissue Specificity: Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon. Post-translational modifications: Autophosphorylated and phosphorylated by IKBKB/IKKB. Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. Contains 1 protein kinase domain. SWISS: Q14164 Gene ID: 9641 Database links: Entrez Gene: 9641 Human Omim: 605048 Human SwissProt: Q14164 Human Unigene: 321045 Human I kappa B kinase epsilon(IKKe)也是絲氨酸/蘇氨酸蛋白激酶家族.可以磷酸化IkB，從而激活NF-kB通路，促進(jìn)NF-kB核移位,并最終降解NF-kB的抑制劑. IKKe可表達(dá)在很多組織細(xì)胞中;胸腺、胰腺、脾、胎盤、外周血白細(xì)胞，也可少量表達(dá)在肺、腎、前列腺癌、卵巢、結(jié)腸。但最多表達(dá)在乳腺腫瘤上，IKBKE在人類乳腺癌中明顯高表達(dá)，這種新的乳腺癌易感基因在腫瘤的形成和發(fā)展中起著及其關(guān)鍵的作用。IKBKE蛋白不同于乳腺癌易感基因BRCA1和BRCA2，是先天具有的。帶有這些遺傳變異的這些基因的女性會增加早發(fā)乳腺癌的風(fēng)險。 |
| 產(chǎn)品圖片 |
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IKBKE/IKKi Polyclonal Antibody, Unconjugated(bs-4114R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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