| 產(chǎn)品編號 | bs-5641R |
| 英文名稱 | phospho-SOX9 (Ser181) Rabbit pAb |
| 中文名稱 | 磷酸化轉(zhuǎn)錄因子SOX9蛋白抗體 |
| 別 名 | SOX9(phospho Ser181); SOX9(phospho S181); p-SOX9(phospho S181); SOX9(phospho S181); CMD 1; CMD1; CMPD 1; CMPD1; SOX 9; Sox9; SOX9_HUMAN; SRA 1; SRA1 antibody SRY(sex determining region Y) box 9(campomelic dysplasia autosomal; SRY(sex determining region Y) |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 發(fā)育生物學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human SOX9 around the phosphorylation site of Ser181: RK(p-S)VK |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Function: Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Subcellular Location: Nucleus (Potential). DISEASE: Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. Defects in SOX9 are the cause of 46,XX sex reversal type 2 (SRXX2) [MIM:278850]. SRXX2 is a condition in which male gonads develop in a genetic female (female to male sex reversal). Similarity: Contains 1 HMG box DNA-binding domain. Gene ID: 6662 Database links: Entrez Gene: 374148 Chicken Entrez Gene: 6662 Human Entrez Gene: 20682 Mouse Omim: 608160 Human SwissProt: P48434 Chicken SwissProt: P48436 Human SwissProt: Q04887 Mouse Unigene: 647409 Human Unigene: 286407 Mouse Sox9是軟骨形成過程中一個(gè)十分關(guān)鍵的轉(zhuǎn)錄因子，雖然已經(jīng)有研究表明骨形成蛋白2(bone morphogenetic protein 2,BMP2)能誘導(dǎo)Sox9的表達(dá)。 有學(xué)者認(rèn)為；Sox9蛋白很可能與椎間盤退變有著密切的關(guān)系，Sox9是膠原蛋白合成過程中的一個(gè)重要的轉(zhuǎn)錄因子，且在軟骨的發(fā)育、成熟過程中對膠原蛋白有著正向調(diào)控作用，Sox9蛋白在男性性腺調(diào)節(jié)睪丸發(fā)育中也有一定的作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-SOX9(Ser181)) Polyclonal Antibody, Unconjugated (bs-5641R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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