| 產(chǎn)品編號 | bs-8429R |
| 英文名稱 | BRPF3 Rabbit pAb |
| 中文名稱 | BRPF3蛋白抗體 |
| 別 名 | BRPF3; BRPF 3; BRPF-3; Bromodomain and PHD finger containing protein 3; KIAA1286; BRPF3_HUMAN. |
| 研究領域 | 細胞生物 免疫學 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 136 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BRPF3: 1101-1205/1205 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
BRPF3 is a 1,205 amino acid protein that forms a complex with MORF, MOZ, ING5, MEAF6 and BRPF. BRPF3 contains one bromo domain, a PHD-type zinc finger and a single PWWP domain. The gene encoding BRPF3 maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6. Function: Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Subunit: Component of the MOZ/MORF complex composed at least of ING5, KAT6A, KAT6B, MEAF6 and one of BRPF1, BRD1/BRPF2 and BRPF3. Similarity: Contains 1 bromo domain. Contains 1 PHD-type zinc finger. Contains 1 PWWP domain. SWISS: Q9ULD4 Gene ID: 27154 Database links: Entrez Gene: 27154 Human Entrez Gene: 268936 Mouse GenBank: NM_015695.1 Human SwissProt: Q9ULD4 Human Unigene: 291877 Mouse Unigene: 214091 Rat |
| 產(chǎn)品圖片 |
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-BRPF3 (bs-8429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 136 kD
Observed band size: 136 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BRPF3) Polyclonal Antibody, Unconjugated (bs-8429R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
