| 產(chǎn)品編號 | bs-13588R |
| 英文名稱 | ZNF575 Rabbit pAb |
| 中文名稱 | 鋅指蛋白575抗體 |
| 別 名 | Zinc finger protein 575; ZN575_HUMAN; Znf575. |
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Specific References (1) | bs-13588R has been referenced in 1 publications.
[IF=4.938] NING AN. et al. The zinc figure protein ZNF575 impairs colorectal cancer growth via promoting p53 transcription. ONCOL RES. 2023; 31(3): 307–316 IHC ; Human,Mouse.
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| 研究領域 | 細胞生物 免疫學 染色質(zhì)和核信號 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF575: 51-150/245 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zinc finger protein 575 (ZNF575) is a 245 amino acid member of the Krüppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZNF575 contains six C2H2-type zinc fingers through which it is thought to be involved in DNA-binding and transcriptional regulation. Function: May be involved in transcriptional regulation. Subcellular Location: Nucleus. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers. SWISS: Q86XF7 Gene ID: 284346 Database links: Entrez Gene: 284346 Human Entrez Gene: 101544 Mouse SwissProt: Q86XF7 Human SwissProt: Q3TXZ1 Mouse Unigene: 213534 Human Unigene: 20117 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF575) Polyclonal Antibody, Unconjugated (bs-13588R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
