| 產(chǎn)品編號 | bs-14009R |
| 英文名稱 | COX6B2 Rabbit pAb |
| 中文名稱 | 細胞色素C氧化酶蛋白6B2抗體 |
| 別 名 | Cancer/testis antigen 59; COX VIb-2; Cox6b2; COXVIb-2; COXVIb2; CT59; CX6B2_HUMAN; Cytochrome c oxidase subunit 6B2; Cytochrome c oxidase subunit VIb; Cytochrome c oxidase subunit VIb isoform 2; cytochrome c oxidase subunit VIb polypeptide 2(testis); Cyto |
| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞類型標志物 新陳代謝 鋅指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Sheep,Cow) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human COX6B2: 1-88/88 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Localized to the intermembrane space, COX6b2 (Cytochrome c oxidase subunit 6B2), also known as Cytochrome c oxidase subunit VIb isoform 2 and Cancer/testis antigen 59, is a 88 amino acid mitochondrial protein that is responsible for joining the two COX monomers to form the COX dimer. COX6b2 is specifically expressed in testis and is found to be upregulated in certain cancer cell lines. Function: Connects the two COX monomers into the physiological dimeric form. Subcellular Location: Mitochondrion intermembrane space. Tissue Specificity: Testis specific. Weak expression in thymus and heart. Expressed in cancer cell lines. Similarity: Belongs to the cytochrome c oxidase subunit 6B family. SWISS: Q6YFQ2 Gene ID: 125965 Database links: Entrez Gene: 125965 Human Entrez Gene: 333182 Mouse SwissProt: Q6YFQ2 Human SwissProt: Q80ZN9 Mouse Unigene: 550544 Human Unigene: 29625 Mouse Unigene: 97699 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX6B2 Polyclonal Antibody, Unconjugated(bs-14009R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX6B2 Polyclonal Antibody, Unconjugated(bs-14009R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
