| 產(chǎn)品編號(hào) | bs-16797R |
| 英文名稱 | KMT4 Rabbit pAb |
| 中文名稱 | 組蛋白H3 K79甲基轉(zhuǎn)移酶抗體 |
| 別 名 | Disrupter of telomere silencing protein 1; DOT 1; DOT1; DOT1 like; DOT1 like histone H3 methyltransferase(S. cerevisiae); DOT1 like histone H3 methyltransferase; DOT1 like protein; DOT1, S. cerevisiae, homolog of; DOT1L; EC 2.1.1.43; H3 K79 HMTase; Histon |
| 研究領(lǐng)域 | 免疫學(xué) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Sheep,Cow,African Green Monkey) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 185 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KMT4: 51-150/1739 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] Function: The KMT4 / Dot1 (disruptor of telomeric silencing) family are a class of histone methyltransferases that play an indirect role in chromatin silencing by targeting a conserved lysine residue in the nucleosome core. Biochemical studies suggest that recombinant Dot1 proteins are active on recombinant nucleosomes, free of any modifications. Subunit: Interacts with MLLT10. Subcellular Location: Nuclear Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. Contains 1 DOT1 domain. SWISS: Q8TEK3 Gene ID: 84444 Database links: Entrez Gene: 84444 Human Omim: 607375 Human SwissProt: Q8TEK3 Human Unigene: 713641 Human Unigene: 228827 Rat |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Liver (Rat) Lysate at 40 ug
Primary: Anti- KMT4 (bs-16797R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 185 kD
Observed band size: 170 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated(bs-16797R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated(bs-16797R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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