| 產品編號 | bs-18029R |
| 英文名稱 | Orexin Receptor 1 Rabbit pAb |
| 中文名稱 | 丘腦分泌素受體1抗體 |
| 別 名 | HCRTR 1; HCRTR1; Hypocretin receptor type 1; Orexin receptor type 1; Ox-1-R; Ox1-R; Ox1r; OX1R_HUMAN. |
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Specific References (3) | bs-18029R has been referenced in 3 publications.
[IF=5.81] Shengyan Xi. et al. The Ciji-Hua’ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma. Front Pharmacol. 2021; 12: 715824 IHC ; Mouse.
[IF=3.333] Hye Ji J. Kim. et al. Dual Cannabinoid and Orexin Regulation of Anhedonic Behaviour Caused by Prolonged Restraint Stress. BRAIN SCI. 2023 Feb;13(2):314 IHC ; Mouse.
[IF=2.751] De-Qi Yan. et al. Establishment of a chronic insomnia rat model of sleep fragmentation using unstable platforms surrounded by water. EXP THER MED. 2023 May;25(5):1-12 IF ; Rat.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Orexin Receptor 1: 101-200/425 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009] Function: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Subcellular Location: Cell membrane. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: O43614 Gene ID: 3062 Database links: Entrez Gene: 3061 Human Entrez Gene: 230777 Mouse Omim: 602392 Human SwissProt: O43613 Human SwissProt: P58307 Mouse Unigene: 388226 Human Unigene: 246595 Mouse Unigene: 88262 Rat |
| 產品圖片 |
Sample: A549 Cell (Human) Lysate at 30 ug
Primary: Anti-Orexin Receptor 1 (bs-18029R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 48kD
Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Pancreas tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Rat Pancreas tissue lysates
Primary: Anti-Orexin Receptor 1 (bs-18029R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
