| 產(chǎn)品編號(hào) | bs-42386R |
| 英文名稱 | P53? Rabbit pAb |
| 中文名稱 | 細(xì)胞腫瘤抗原P53抗體 |
| 別 名 | P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53; |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測(cè)分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human P53 protei: 1-393/393 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]. SWISS: P04637 Gene ID: 7157 wtp53廣泛的研究發(fā)現(xiàn)P53腫瘤抑制基因?qū)?0%以上的人類癌癥具有抑制突變的功能。P53蛋白水平在正常細(xì)胞中表達(dá)低，在DNA突變時(shí)或各種各樣細(xì)胞遇難信號(hào)時(shí)反應(yīng)增加。該基因突變或缺失是導(dǎo)致許多腫瘤發(fā)生的原因。 野生型P53(wt-p53)可誘導(dǎo)細(xì)胞凋亡，并通過(guò)細(xì)胞凋亡抑制腫瘤生長(zhǎng)，而P53的突變或缺失則可抑制野生型P53的功能，使得缺陷細(xì)胞得以存活下來(lái)，從而導(dǎo)致腫瘤發(fā)生。 P53同時(shí)也是細(xì)胞凋亡的調(diào)控因子。此抗體可用于P53腫瘤抑制基因功能的研究。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with P53 polyclonal antibody, unconjugated (bs-42386R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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