| 產品編號 | bs-8537R-Gold |
| 英文名稱 | Rabbit Anti-Thymidylate synthase/Gold Conjugated antibody |
| 中文名稱 | 膠體金標記的胸苷酸合成酶抗體 |
| 別 名 | d TMP synthase; EC 2.1.1.45; HsT422; MGC88736; Thymidylate synthase; thymidylate synthetase; TMS; TS; TSase; TYMS; TYMS protein; Tyms thymidylate synthetase; TYSY_HUMAN . |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul（10nm 15nm 35nm） |
| 研究領域 | 腫瘤 細胞生物 免疫學 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep, ) |
| 產品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 36kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Thymidylate synthase/TS |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產品介紹 |
background: Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time. Function: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Subunit: Homodimer. Subcellular Location: Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. Similarity: Belongs to the thymidylate synthase family. Database links: UniProtKB/Swiss-Prot: P04818.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 胸苷酸合成酶(thymidylate synthase,TS)是生物體內催化胸苷酸合成所必需的酶,多年來一直作為腫瘤化療的重要靶酶。對TS基因調控機制的研究表明:基因擴增、轉錄、翻譯和翻譯后過程都參與了TS表達的調控。TS可與自身的mRNA結合形成TS-mRNA復合物,使mRNA翻譯受阻,5-氟尿嘧啶(5-fluorouracil,5-FU)等抗代謝藥物可與TS蛋白結合,結合后的復合物不能與TS mRNA作用,導致體內TS的表達升高,是腫瘤細胞產生抗藥性的重要分子機制之一。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
